Isolation and identification of stem cells derived from human exfoliated deciduous teeth / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To isolate and identify stem cells from human exfoliated deciduous teeth (SHED).</p><p><b>METHODS</b>Human pulp tissue were dissected and digested to obtain the single cell suspension. The cell morphology was observed and the clonality of the obtained cells was assessed. The phenotype of the cells was detected by immunohistochemistry and flow cytometry (FCM), and the cell cycle was analyzed. The in vitro differentiation of the cells into adipose tissue and formation of mineralization nodules were evaluated.</p><p><b>RESULTS</b>Clonogenic assay showed the formation of 16-18 clones in every 10(3) plated cells derived from human exfoliated deciduous teeth. These cells were found to express the markers of mesenchymal stem cells with a multipotent differentiation potential.</p><p><b>CONCLUSION</b>The cells isolated from human dental pulp are clonogenic and have multipotent differentiation potential, suggesting their identity of SHED.</p>