Detection of CD95 and CD95L mRNA expression after liver transplantation using SYBR real-time PCR during acute rejection / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To detect the expression of CD95 and CD95L mRNAs after liver transplantation and investigate the role of CD95 and CD95L in acute liver allograft rejection.</p><p><b>METHODS</b>The expressions of CD95 and CD95L mRNAs of peripheral blood lymphocyte from 56 liver allograft recipients were examined using SYBR real-time PCR.</p><p><b>RESULTS</b>CD95 and CD95L mRNA levels in the recipients with acute rejection were significantly higher than those without rejection (P<0.01), and the elevation occurred about 2 days earlier than that of alanine aminotransferase and aspartate aminotransferase.</p><p><b>CONCLUSION</b>CD95 and CD95L are related to acute liver allograft rejection and their mRNA expression level may serve as an indicator for prediction and diagnosis of acute rejection episodes.</p>