Application of fluorescence in situ hybridization technique in diagnosis of variant Ph chromosome translocation and Ph-negative chronic myelocytic leukemia / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 563-566, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-234360
ABSTRACT
<p><b>OBJECTIVE</b>To explore the value of fluorescence in situ hybridization (FISH) technique in diagnosis of variant Ph chromosome translocation (VT) and Ph chromosome-negative chronic myelocytic leukemia (CML).</p><p><b>METHODS</b>Nine CML patients with VT and 2 Ph chromosome-negative CML patients confirmed by R banding were assayed with dual color-dual fusion BCR/ABL probe by FISH.</p><p><b>RESULTS</b>The 9 patients with VT involved chromosomes 1, 3, 5, 12, 13, 15, 17 and 21 besides chromosomes 9 and 22, and some of them showed recurrent aberrations; FISH results were positive and the signal feature was 2R2G1Y. The 2 Ph-negative CML patients had normal karyotypes; FISH was positive and the signal feature was 1R1G2Y and 1R1G1Y respectively.</p><p><b>CONCLUSION</b>FISH can provide better diagnosis for CML with VT and Ph-negative CML. Abnormal karyotype and marker gene changes can be assessed based on the signal feature of the positive cell. So FISH is a complementary method to banding technique in diagnosis of CML.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Translocation génétique
/
Chromosome Philadelphie
/
Leucémie myéloïde chronique BCR-ABL positive
/
Protéines de fusion bcr-abl
/
Chromosomes
/
Hybridation fluorescente in situ
/
Diagnostic
/
Leucémie myéloïde chronique atypique BCR-ABL négative
/
Génétique
/
Méthodes
Type d'étude:
Etude diagnostique
Limites du sujet:
Adulte
/
Adulte très âgé
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Medical Genetics
Année:
2010
Type:
Article
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