Effects of TCM treatment according to syndrome differentiation on expressions of nuclear factor-kappaB and gamma-glutamylcysteine synthetase in rats with chronic obstructive pulmonary disease of various syndrome types / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 426-430, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-234752
ABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism of traditional Chinese medicine (TCM) treatment according to syndrome differentiation in treating chronic obstructive pulmonary disease (COPD) by observing the changes of nuclear factor-kappaB (NF-kappaB) and gamma-glutamylcysteine synthetase (gamma-GCS) expression levels in rats.</p><p><b>METHODS</b>COPD model was established by modified method of combining fumigation and lipopolysaccharide (LPS) intra-tracheal dripping. Model rats were treated respectively for succesive 14 days according to their syndrome, that is, Xiaoqinglong Decoction to the rats of cold-phlegm accumulation in Fei, Maxing Shigan Decoction to those of heat-phlegm accumulation in Fei, Yupingfeng Decoction to those of Fei-qi deficiency, Liujunzi Decoction to those of Pi-qi deficiency, Renshen Gejie Decoction to those of Shen qi-deficiency. Besides, model rats in the model control group received 2mL normal saline daily, and no intervention was applied in the normal control group. The expression of gamma-GCS and NF-kappaB was detected by immunochemistry before and after treatment.</p><p><b>RESULTS</b>Compared with that in the normal rats, the expressions of gamma-GCS and NF-kappaB in bronchial and alveolar epithelium of COPD rats before treatment were significantly higher, but the positive expression rates were lowered after treatment significantly (P<0.05).</p><p><b>CONCLUSION</b>TCM treatment according to syndrome differentiation could rectify imbalance of oxidation/anti-oxidation and alleviate inflammatory reaction in COPD rats, thus to treat COPD effectively.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Syndrome
/
Médicaments issus de plantes chinoises
/
Immunohistochimie
/
Répartition aléatoire
/
Facteur de transcription NF-kappa B
/
Rat Wistar
/
Broncho-pneumopathie chronique obstructive
/
Utilisations thérapeutiques
/
Diagnostic
/
Diagnostic différentiel
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Integrated Traditional and Western Medicine
Année:
2007
Type:
Article
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