Experimental study of the A549 cell apoptosis of human non-small cell lung cancer induced by ginseng polysaccharide in vitro / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the in vitro effect of Ginseng polysaccharide on the proliferation and apoptosis of human non-small cell lung cancer.</p><p><b>METHODS</b>The apoptosis of A549 cell induced by Ginseng polysaccharide was detected by MTT method, flow cytometry, Annexin V-PI double-labeling, Hoechest luorescent staining and TUNEL. The change of bcl-2 expression after Ginseng polysaccharide treatment was observed by immunohistochemical method.</p><p><b>RESULTS</b>Ginseng polysaccharide had inhibitive action on A549 cell growth, and apoptosis promoting effect as well. Its effect on cell cycle was manifested as G0/G1 phase arrest. No down-regulation of bcl-2 expression was seen. Morphological changes of apoptosis could be observed under electron microscope, Hoechst and TUNEL techniques.</p><p><b>CONCLUSION</b>Ginseng polysaccharide could inhibit the growth of human non-small cell lung cancer A549 cell,and induce the cell apoptosis,which might be one of the anti-cancer mechanisms of Ginseng polysaccharide. The occurrence of cell apoptosis had no clear relationship with the expression of bcl-2.</p>