Experimental research on effect of human mesenchymal stem cells induced by shenqi fuzheng injection in cerebral infarction / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 629-632, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-234809
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of shenqi fuzheng injection (SFI) in inducing differentiation of human mesenchymal stem cells (hMSCs) in brain stem and its effect on nervous function in model rats of cerebral infarction.</p><p><b>METHODS</b>Middle cerebral artery occlusion model rats were made, and hMSCs was injected into their brain after being amplified in vitro and incubated with SFI for 0.5 h, then the survival, migration and differentiation of hMSCs in brain stem as well as the change of nervous function in model rats were observed.</p><p><b>RESULTS</b>The post-transplantation reject reaction to hMSCs was low, it could survive as long as 6 weeks or more. No difference in area of infarction was shown before and after transplantation. Immunohistochemical staining showed that hMSCs expressed human neuron specific enolase (NSE), neurofilament (NF) and glial fibrillary acid protein (GFAP). The limb-kinetic function and tactile perception were improved in the model rats.</p><p><b>CONCLUSION</b>SFI can induce hMSCs differentiate into neurons in vivo, and hMSCs may be the ideal germinal cells for treating cerebral infarction.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Enolase
/
Chirurgie générale
/
Transplantation hétérologue
/
Encéphale
/
Médicaments issus de plantes chinoises
/
Différenciation cellulaire
/
Division cellulaire
/
Cellules cultivées
/
Infarctus cérébral
Limites du sujet:
Animaux
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Integrated Traditional and Western Medicine
Année:
2005
Type:
Article
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