Effect of hypoxia on gastric cancer cell proliferation and LINE-1 endonuclease variant GCRG213 expression / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 225-228, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-235394
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the change of gastric cancer cell proliferation and the expression of gastric cancer related gene 213 (GCRG213), a long interspersed nuclear element-1 (LINE-1) endonuclease variant, during hypoxia.</p><p><b>METHODS</b>Normal gastric mucosa cell GES-1 and gastric cancer cell BGC-823 were cultured in 20% or 3% oxygen concentrations, respectively. MTT test was used to analyze the proliferation of the GES-1 and BGC-823 cells. The change of GCRG213 mRNA and protein expression in GES-1 and BGC-823 cells was detected by using RT-PCR and Western blot analysis. Blast was used at the NCBI Blast server to identify GCRG213 sequence to any alignment in the GeneBank databases.</p><p><b>RESULTS</b>Compared with 20% oxygen condition, 3% oxygen concentration could promote cell growth. Mean-while, the expression of GCRG213 at mRNA and protein levels was increased. GCRG213 sequence shared high homology with LINE-1 endonuclease sequence.</p><p><b>CONCLUSION</b>GCRG213 is a variant of LINE-1 endonuclease. Hypoxia as in 3% oxygen condition can promote cell proliferation and lead to GCRG213 overexpression.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Tumeurs de l'estomac
/
Expression des gènes
/
Cellules cultivées
/
Biologie cellulaire
/
Hormones peptidiques
/
Lignée cellulaire tumorale
/
Deoxyribonuclease I
/
Prolifération cellulaire
/
Muqueuse gastrique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2013
Type:
Article
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