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Altered expression of matrix metalloproteinases and tight junction proteins in rats following PEMF-induced BBB permeability change / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 197-202, 2012.
Article Dans Anglais | WPRIM | ID: wpr-235535
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of occludin, ZO-1, MMP-2, and MMP-9 in cerebral microvasculature following Pulse Electromagnetic Field (PEMF) induced BBB permeability change.</p><p><b>METHODS</b>Sprague-Dawley rats were randomized into PEMF and sham exposed groups (n = 8). After exposure to PEMF at 0.5, 1, 3, 6, and 12 h, BBB permeability was measured by Evans-Blue extravasation. The expression of occludin, ZO-1, MMP-2, and MMP-9 were detected by real-time quantitative reverse transcriptase PCR and western blotting. MMP-2 and MMP-9 activity were detected by EnzChek gelatinase assay.</p><p><b>RESULTS</b>Compared with the sham group, PEMF exposure led to increased permeability of the BBB to EB, which was prolonged after exposure. BBB permeability became progressively more severe, and recovered at 6 h. The gene and protein expression of occludin and ZO-1 were significantly decreased, while MMP-2 and MMP-9 expression were significantly increased after exposure to PEMF. All levels of expression recovered 12 h following PEMF.</p><p><b>CONCLUSION</b>Changes to BBB permeability were related to the alteration expression of tight junction proteins and matrix metalloproteinase after exposure to PEMF.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Barrière hémato-encéphalique / Protéines / Rat Sprague-Dawley / Jonctions serrées / Matrix metalloproteinases / Champs électromagnétiques / Métabolisme Limites du sujet: Animaux langue: Anglais Texte intégral: Biomedical and Environmental Sciences Année: 2012 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Barrière hémato-encéphalique / Protéines / Rat Sprague-Dawley / Jonctions serrées / Matrix metalloproteinases / Champs électromagnétiques / Métabolisme Limites du sujet: Animaux langue: Anglais Texte intégral: Biomedical and Environmental Sciences Année: 2012 Type: Article