Preparation and initial application of a monoclonal antibody specific for a newly discovered conserved linear epitope of rabies virus nucleoprotein / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 98-103, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-235565
ABSTRACT
<p><b>OBJECTIVE</b>To prepare monoclonal antibodies against a newly discovered and conserved linear epitope of Rabies virus nucleoprotein and to use them in a rabies diagnostic test.</p><p><b>METHODS</b>Synthetic peptide containing the epitope was used as immunogen to prepare hybridoma cell lines by classical hybridoma technology. Anti-peptide monoclonal antibodies produced in ascites of inoculated Balb/c mice were labeled with fluorescein isothiocyanate (FITC) after purification and used in fluorescent antibody test (FAT).</p><p><b>RESULTS</b>Two positive hybridoma cell lines, RVNP-mAb1-CL and RVNP-mAb2-CL, were obtained. RVNP- mAb1-CL produced a higher concentration of monoclonal antibody RVNP-mAb1 in Balb/c ascites. FITC-labeled RVNP-mAb1 showed correct results on certain Rabies virus-positive canine brain tissue samples and cells of a small subclone of baby hamster kidney 21 cell line (BSR).</p><p><b>CONCLUSION</b>FITC-labeled RVNP-mAb1 has potential application for laboratory diagnosis of rabies.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virus de la rage
/
Protéines virales
/
Lignée cellulaire
/
Fluorescéine-5-isothiocyanate
/
Allergie et immunologie
/
Colorants fluorescents
/
Hybridomes
/
Souris de lignée BALB C
/
Anticorps monoclonaux
/
Épitopes
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Biomedical and Environmental Sciences
Année:
2012
Type:
Article
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