Study on application of amphotericin B in the perforated whole-cell patch clamp technique / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 33-36, 2014.
Article
de Zh
| WPRIM
| ID: wpr-236393
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Established with amphotericin B perforated patch-clamp technique, to study the electrophysiological properties of calyx synapses.</p><p><b>METHODS</b>In the present experiments, we studied the application of perforated patch clamp technique on the calyx synapses of mice with Amphotericin B.</p><p><b>RESULTS</b>The use of Amphotericin B significantly slowed down the decay of channel currents and the optimum concentration was 400 microg/ml.</p><p><b>CONCLUSION</b>The syudy developed a stable of perforated patch clamp whole cell recording technique, could be more effecitve, more real reaction neurons electrophysiological characteristics of the channel current. Our work might provide the basic information to future users studying the signal transmission and regulation of auditory system of rodents.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
/
Amphotéricine B
/
Techniques de patch-clamp
/
Neurones
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2014
Type:
Article