Effect of shenmai injection on L-type calcium current of diaphragmatic muscle in rats / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 376-378, 2004.
Article
de En
| WPRIM
| ID: wpr-236519
Bibliothèque responsable:
WPRO
ABSTRACT
In this study, whole cell patch clamp recording technique was employed to investigate the effect of Shenmai Injection (SMI) on L-type calcium current of diaphragmatic muscle in rats. The result showed that when the diaphragmatic muscle cell was held at -80 mV and depolarized to +60 mV, 10 microl/ml, 50 microl/ml and 100 microl/ml SMI enhanced the inner peak L-type calcium current from -(6.8 +/- 0.7) pA/pF (n=7) to -(7.3 +/- 0.8) pA/pF (P>0.05, n=7), -(8.6 +/- 1.0) pA/pF (P<0.05, n=7) and -(9.4 +/- 1.2) pA/pF (P<0.05, n=7), respectively, The rates of L-type calcium current were increased by (7.34 +/- 2.37)%, (25.72 +/- 5.94)%, and (38.16 +/- 7.33)%, respectively. However, it had no significant effect on maximal activation potential and reversal potential. Our results suggested that SMI could activate the calcium channel of the diaphragmatic fibers of the rats, increase the influx of Ca2+, and enhance the contractility of diaphragmatic muscles.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Muscle diaphragme
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Médicaments issus de plantes chinoises
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Extraits de plantes
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Calcium
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Rat Wistar
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Techniques de patch-clamp
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Canaux calciques de type L
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Association médicamenteuse
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Métabolisme
Limites du sujet:
Animals
langue:
En
Texte intégral:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Année:
2004
Type:
Article