Knocking down osteopontin expression by specific siRNA reduces the in vitro invasiveness of human hepatocellular carcinoma cells / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 404-407, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-236930
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of osteopontin (OPN) expression down-regulated by RNA interference (RNAi) on the invasiveness of hepatocelluar carcinoma cell line HCC-LM3.</p><p><b>METHODS</b>HCC-LM3 cells were transfected with the chemically synthesized small interfering RNA (siRNA) formulated by lipofectamine 2000. Wild type HCC-LM3 and HCC-LM3 cells transfected with non-specific siRNA served as controls. Real-time PCR and Western blotting were used to quantify the mRNA and OPN protein levels. The malignant phenotypes of transfected HCC-LM3 cells including cellular growth rate, colony formation and Matrigel invasion activities were analyzed.</p><p><b>RESULTS</b>Sequence-specific siRNAs targeting OPN suppressed OPN RNA expression by 79% and also decreased OPN protein level by 81% in HCC-LM3 cells. The number of formed colonies and migrating numbers in vitro were decreased in HCC-LM3 cells transfected using sequence-specific siRNAs targeting OPN relative to controls (P < 0.05).</p><p><b>CONCLUSION</b>This study demonstrated that specific siRNA is able to reduce OPN at both the mRNA and protein levels and significantly diminishes the invasiveness of hepatocellular carcinoma cells.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
ARN messager
/
Transfection
/
Régulation négative
/
Mouvement cellulaire
/
Carcinome hépatocellulaire
/
Petit ARN interférent
/
Interférence par ARN
/
Lignée cellulaire tumorale
/
Prolifération cellulaire
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Oncology
Année:
2006
Type:
Article
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