Isolation and characterization of a DRE-binding transcription factor from Yinxin poplar (Populus alba x P. alba var. pyramidalis) / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 906-910, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-237052
ABSTRACT
Dehydration-Responsive Element Binding ( DREB) transcription factors, specifically binding with dehydration reponsive element (DRE), activate a variety of stress-responsive genes in plants under abiotic stresses (dehydration, high salt and low temperature). Using PCR and homologous EST search, we isolated a DREB-like gene from Yinxin poplar (Populus alba x P. alba var. pyramidalis) named PaDREB2. Yeast One-hybrid experiment demonstrated that PaDREB2 protein could function as a DREB transcription factor activating target gene expression by specifically binding to DRE cis-element. To study the expression pattern of PaDREB2, RT-PCR was carried out. And the results showed that PaDREB2 is induced by low temperature, drought and high salt.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phylogenèse
/
Protéines végétales
/
Stress physiologique
/
Facteurs de transcription
/
Données de séquences moléculaires
/
Séquence d'acides aminés
/
Clonage moléculaire
/
Basse température
/
Régulation de l'expression des gènes végétaux
/
Étiquettes de séquences exprimées
Type d'étude:
Étude pronostique
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2005
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS