Expression of FOS protein in glioma and its effect on the growth of human glioma cells / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the expression of FOS protein in human glioma tissues and its effect on tumor growth.</p><p><b>METHODS</b>FOS protein expression in glioma tissues was determined with immunohistochemical (IHC) staining. Subsequently, 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay, flow cytometry, transwell invasion and Western blotting were used to assay U87 and U251 cells with reduced FOS expression.</p><p><b>RESULTS</b>The expression of FOS in glioma was increased and strongly correlated with its pathological grade. Abrogating expression of FOS has suppressed proliferation and invasion, and delayed cell cycle at G1 phrase for both U87 and U251 cells.</p><p><b>CONCLUSION</b>The expression of FOS protein in human glioma was strong. FOS protein probably plays a critical role in the progression of gliomas.</p>