Active ingredients and its pharmacokinetic behavior and anti-inflammatory effects of ginseng with different steamed times / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 3770-3774, 2015.
Article
de Zh
| WPRIM
| ID: wpr-237733
Bibliothèque responsable:
WPRO
ABSTRACT
HPLC analysis was performed to study the changes in chemical composition of ginseng extracts prepared from high quality ginseng with 0, 2, 4, 8 h of steamed times. An UFLC-MS/MS multiple-reaction monitoring (MRM) quantitative analysis was made to investigate the pharmacokinetic behavior differences of ginsenosides in mice ig administered of ginseng extracts with different steamed times in the negative ion mode, with Digoxin as the internal standard substance. The mice were injected with LPS to establish inflammation model after ig administration of ginseng for a week and the contents of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in mice plasma were detected by ELISA, in order to study on anti-inflammatory effects of ginseng with different steamed times. It was determined that levels of TNF-α and IL-1β were significantly decreased in inflammation model group ig administered of ginseng extracts with 8h of steamed time. The results showed that the chemical components in ginseng changed after steaming and the components into the blood changed, correspondingly. Ginseng with steamed 8 h contributes to anti-inflammatory effects. These results provided an experimental basis for revealing the active substance basis and dose-effect relationship of ginseng on anti-inflammatory effect.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Facteurs temps
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Médicaments issus de plantes chinoises
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Pharmacocinétique
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Chimie
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Chimie pharmaceutique
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Ginsénosides
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Traitement médicamenteux
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Panax
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Température élevée
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Inflammation
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
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Humans
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Male
langue:
Zh
Texte intégral:
Zhongguo Zhong Yao Za Zhi
Année:
2015
Type:
Article