C-reactive protein induced human endothelium cells apoptosis is associated with Bcl-2/Bax gene expression changes / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 639-642, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-238546
ABSTRACT
<p><b>OBJECTIVE</b>In the present study, we examined the expression changes of Bcl-2/Bax in C-reactive protein (CRP) treated human endothelium cells in vitro.</p><p><b>METHODS</b>The human umbilical vein endothelial cells (HUVEC) were cultured by digest method for 2 - 3 posterities and incubated with human CRP (0, 1, 5, 25 mg/L for 24 hours) and analyzed by flow cytometer for apoptosis ratio. The effects of CRP in various concentrations on Bcl-2/Bax mRNA and protein expression were examined by RT-PCR and Western Blotting.</p><p><b>RESULTS</b>Apoptosis ratio increased, downregulated Bcl-2 (gene promoting cell survival) and upregulated Bax (gene promoting apoptosis) at mRNA and protein levels in proportion to increased CRP concentrations.</p><p><b>CONCLUSION</b>These results demonstrate that Bcl-2/Bax could be regulated by CRP in human HUVECs and might play a causal role in CRP-induced apoptosis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Veines ombilicales
/
Protéine C-réactive
/
Lignée cellulaire
/
Apoptose
/
Protéines proto-oncogènes c-bcl-2
/
RT-PCR
/
Biologie cellulaire
/
Cellules endothéliales
/
Protéine Bax
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Cardiology
Année:
2006
Type:
Article
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