Expression of miR-223 in clear cell renal cell carcinoma and its significance / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the expression of miR-223 in clear cell renal cell carcinoma (ccRcc) and its clinical implications.</p><p><b>METHODS</b>Quantitative real-time PCR was employed to detect the levels of miR- 223 expression in ccRcc, pair-matched adjacent normal tissues and different renal cancer cell lines. Transwell migration essay and wound healing essay were used to evaluate the invasion and migration of renal cancer 786-O cells transfected with miR-223 mimics. MTT essay was used to measure the cell proliferation, and the cell cycle changes following the transfection were analyzed with flow cytometry.</p><p><b>RESULTS</b>Compared with the normal tissues, the cancer samples showed up-regulated miR-223 expression, which was associated with tumor size. In 786-O cell cultures, transfection with miR-223 mimics significantly enhanced cell migration (P<0.0001) and growth (P=0.006) and induced G1 cell cycle arrest.</p><p><b>CONCLUSION</b>miR-223 promotes renal cancer cell migration and proliferation and may serve as a potential therapeutic target for ccRcc.</p>