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Expression and significance of miR-125a and Mcl-1 in intestinal tissue after massive small bowel resection in rat / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery ; (12): 495-498, 2014.
Article Dans Chinois | WPRIM | ID: wpr-239370
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression and significance of miR-125a and anti-apoptotic protein Mcl-1 in intestinal tissue after massive small bowel resection in intestinal adaptation.</p><p><b>METHODS</b>Sprague-Dawley rats (54 male rats, 8-week old) were divided into 3 groups randomly, including two control groups. Rats in the experiment group were subjected to 70% massive small bowel resection. Rats in the resection group underwent simple intestinal resection and anastomosis. Rats in the control group underwent laparotomy alone. A 5 cm intestine approximately 1 cm distal to the anastomosis was harvested a week after operation. Expression of Mcl-1 was assessed by immunohistochemistry and real-time PCR was used to detect the expression of miR-125a in intestinal tissue.</p><p><b>RESULTS</b>The positive expression of Mcl-1 in the experiment group was 18.8%(3/16), significantly lower than that in the control group(76.5%, 13/17) and the resection group (83.33%, 15/18)(both P<0.01). The expression of miR-125a in the experiment group was 1.92, significantly higher than that in the control group (1.01) and the resection group (1.05)(both P<0.01).</p><p><b>CONCLUSION</b>miR-125a and anti-apoptotic protein Mcl-1 may play an important role in intestinal adaptation process and they may regulate each other through a certain pathway.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Syndrome de l&apos;intestin court / Chirurgie générale / Anastomose chirurgicale / Rat Sprague-Dawley / MicroARN / Modèles animaux de maladie humaine / Protéine Mcl-1 / Intestin grêle / Métabolisme Type d'étude: Étude pronostique Limites du sujet: Animaux langue: Chinois Texte intégral: Chinese Journal of Gastrointestinal Surgery Année: 2014 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Syndrome de l&apos;intestin court / Chirurgie générale / Anastomose chirurgicale / Rat Sprague-Dawley / MicroARN / Modèles animaux de maladie humaine / Protéine Mcl-1 / Intestin grêle / Métabolisme Type d'étude: Étude pronostique Limites du sujet: Animaux langue: Chinois Texte intégral: Chinese Journal of Gastrointestinal Surgery Année: 2014 Type: Article