Overexpression of PPARγ induces adipogenic steatosis in mouse primary hepatocytes / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 68-74, 2016.
Article
Dans Chinois
| WPRIM
| ID: wpr-239620
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of PPARγ overexpression on steatosis in mouse primary hepatocytes.</p><p><b>METHODS</b>Primary hepatocytes isolated from C57BL/6J mice were infected with either Ad/LacZ or Ad/PPARγ for 48 h. Steatosis of the primary hepatocytes was checked by Oil Red O staining. The mRNA and protein expression of adipocyte-specific genes PPARγ, aP2 and CideA were analyzed by using RT Real-time PCR and Western Blot.</p><p><b>RESULTS</b>Primary hepatocytes were small and even. Hepatocyte nuclei were round with dispersed chromatin and prominent nucleoli. Accumulated lipid droplets were observed in Ad/PPARγ-infected hepatocytes, but in Ad/LacZ-infected hepatocytes. Moreover, compared with Ad/LacZ-infected hepatocytes, the mRNA expression of PPARγ, aP2, FGF21 and CideA in Ad/PPARγ-infected hepatocytes were significantly induced, the protein expression of PPARγ and its target aP2 strongly increased.</p><p><b>CONCLUSION</b>over expression of PPARγ induces adipogenic steatosis in mouse primary hepatocytes.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Transfection
/
Cellules cultivées
/
Adipocytes
/
Hépatocytes
/
Récepteur PPAR gamma
/
Adipogenèse
/
Stéatose hépatique
/
Vecteurs génétiques
/
Métabolisme
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2016
Type:
Article
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