Interaction of Flightless I with Nup88 and Importin β / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1247-1254, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-240559
ABSTRACT
High expression of Fightless I (FLII) is associated to multiple tumors. Based on our previous study that FLII might be involved in the nuclear export, we assessed the possible interaction of FLII with the nuclear envelop associating proteins Importin β and Nup88. We first constructed GST-FLII, GST-LRR recombinant plasmids and transformed them into the Rosetta strain to produce GST-FLII, GST-LRR fusion protein. After purification of these proteins, GST-pull down, as well as co-immunoprecipitation, were used to test the interaction of FLII with Importin β and Nup88. FLII interacted with Importin β and Nup88, and FLII LRR domain is responsible for these interactions. Thus, FLII may play a role in nuclear export through interaction with Importin β and Nup88.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines de fusion recombinantes
/
Récepteurs cytoplasmiques et nucléaires
/
Caryophérines bêta
/
Complexe protéique du pore nucléaire
/
Métabolisme
/
Protéines des microfilaments
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2015
Type:
Article
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