Progress in transcriptional studies / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1141-1150, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-240569
ABSTRACT
Gene expression exhibits temporal and spatial patterns to response environmental changes and growth cycle. Gene expression is under strict control at different levels among which control at transcription level is the predominant mode, especially in prokaryotes. In this review, we summarized the new developments of methods used in transcriptional studies, including modifications and improvements of the classic methods, such as gel-shift assay, DNA foot printing, and in vivo reporter system. In addition, we introduced examples to apply new methods, such as surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) to characterize protein-DNA, ligand-protein, and ligand-protein-DNA interactions. The collection of these methods and their application could guide and accelerate relevant studies.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Transcription génétique
/
Calorimétrie
/
Protéines
/
Expression des gènes
/
Prise d'empreintes sur l'ADN
/
Résonance plasmonique de surface
/
Ligands
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2015
Type:
Article
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