Optimization and application of targeted LC-MS metabolomic analyses in photosynthetic cyanobacteria / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 577-590, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-240617
ABSTRACT
To accurately analyze metabolites in industry-important photosynthetic microbes, LC-MS based metabolomics protocol needs to be optimized specifically for individual species. In this study, an LC-MS based metabolomics method was optimized for cyanobacterium Synechocystis sp. PCC 6803. With the optimized extraction, liquid chromatographic and mass spectral parameters, the method was capable of detecting 24 important metabolites related to central carbohydrate and energy metabolism in Synechocystis sp. PCC 6803. The study laid an important foundation for the metabolomics analysis of cyanobacteria.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Photosynthèse
/
Spectrométrie de masse
/
Chromatographie en phase liquide
/
Synechocystis
/
Métabolome
/
Métabolomique
/
Métabolisme
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2015
Type:
Article
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