Succinic acid production from sucrose and sugarcane molasses by metabolically engineered Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
; (12): 534-541, 2015.
Article
de Zh
| WPRIM
| ID: wpr-240621
Bibliothèque responsable:
WPRO
ABSTRACT
Sugarcane molasses containing large amounts of sucrose is an economical substrate for succinic acid production. However, Escherichia coli AFP111 cannot metabolize sucrose although it is a promising candidate for succinic acid production. To achieve sucrose utilizing ability, we cloned and expressed cscBKA genes encoding sucrose permease, fructokinase and invertase of non-PTS sucrose-utilization system from E. coli W in E. coli AFP111 to generate a recombinant strain AFP111/pMD19T-cscBKA. After 72 h of anaerobic fermentation of the recombinant in serum bottles, 20 g/L sucrose was consumed and 12 g/L succinic acid was produced. During dual-phase fermentation comprised of initial aerobic growth phase followed by anaerobic fermentation phase, the concentration of succinic acid from sucrose and sugarcane molasses was 34 g/L and 30 g/L, respectively, at 30 h of anaerobic phase in a 3 L fermentor. The results show that the introduction of non-PTS sucrose-utilization system has sucrose-metabolizing capability for cell growth and succinic acid production, and can use cheap sugarcane molasses to produce succinic acid.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Protéines de transport membranaire
/
Saccharose
/
Mélasses
/
Chimie
/
Bioréacteurs
/
Acide succinique
/
Protéines Escherichia coli
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Saccharum
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Escherichia coli
/
Fermentation
langue:
Zh
Texte intégral:
Chinese Journal of Biotechnology
Année:
2015
Type:
Article