Verapamil and hypothermia protect spermatogenesis of torsioned testes in rats / 中华男科学杂志
National Journal of Andrology
;
(12): 796-800, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-241254
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effect of verapamil and hypothermia on the spermatogenesis of rats after testicular torsion.</p><p><b>METHODS</b>Sixty healthy pubertal male Sprague-Dawley rats were equally divided into 5 groups A (testis torsion), B (testis torsion + verapamil), C (testis torsion + hypothermia), D (testis torsion + verapamil + hypothermia) and E (control). After treatment, the left testis was removed for the observation of the histological changes under the microscope and measurement of the percentage of apoptotic cells by flow cytometry.</p><p><b>RESULTS</b>HE staining showed disordered arrangement, reduced layers and decreased number of spermatogenic cells, apoptotic bodies, necrosis and partial invasion of inflammatory cells in all the groups but E, most obvious in Group A. The apoptosis rates of germ cells in Groups A, B, C, D and E were (32.11 +/- 2.20)%, (20.18 +/- 1.50)%, (20.02 +/- 1.90)%, (13.75 +/- 1.40)% and (8.56 +/- 0.90)%, respectively, and the Makler scores in the 5 groups were (14.47 +/- 1.35), (15.45 +/- 0.75), (15.48 +/- 0.75), (16.22 +/- 0.72) and (19.60 +/- 0.56), respectively, both with statistically significant differences (P < 0.01).</p><p><b>CONCLUSION</b>The increased apoptosis of germ cells after testicular torsion-and-reposition may reduce the spermatogenesis of the testis. Either verapamil or local hypothermia can enhance testicular resistance against injuries, and the combination of the two can more efficiently prevent the germ cells from apoptosis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Torsion du cordon spermatique
/
Spermatogenèse
/
Thérapeutique
/
Vérapamil
/
Lésion d'ischémie-reperfusion
/
Rat Sprague-Dawley
/
Apoptose
/
Utilisations thérapeutiques
/
Hypothermie provoquée
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
National Journal of Andrology
Année:
2009
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS