Expression of high mobility group protein A in male mouse testicular cell lines / 中华男科学杂志
National Journal of Andrology
;
(12): 534-537, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-241305
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of high mobility group protein A (HMGA) in male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts), and to pave the theoretical ground for further investigation of the action mechanism of the HMGA gene in male mouse spermatogenesis.</p><p><b>METHODS</b>We detected the expressions of HMGA1 and HMGA2 by RT-PCR and Western blot in the male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts).</p><p><b>RESULTS</b>HMGA1 and HMGA2 were expressed in the male mouse testicular cell lines TM4, GC-1spg and GC-2spd(ts) at both mRNA and protein levels. Western blot and RT-PCR methods showed similar results.</p><p><b>CONCLUSION</b>The expression of HMGA may be involved in the cell division and proliferation of TM4, GC-1spg and GC-2spd(ts) and play an important role in spermatogenesis of male mice.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Spermatogenèse
/
Testicule
/
ARN messager
/
Expression des gènes
/
Division cellulaire
/
Lignée cellulaire
/
RT-PCR
/
Biologie cellulaire
/
Protéine HMGA1a
/
Protéine HMGA2
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
National Journal of Andrology
Année:
2009
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS