Preparation of solid lipid nanoparticles loaded with all-trans retinoic acid and their evaluation in vitro and in vivo / 药学学报
Acta Pharmaceutica Sinica
;
(12): 71-75, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-241330
ABSTRACT
<p><b>AIM</b>To prepare solid lipid nanoparticles (SLN) loaded with all trans retinoic acid using an ultrasonic technique with Compritol 888 ATO as matrix material, and investigate properties of nanoparticles in vitro and in vivo.</p><p><b>METHODS</b>Ultrasonic technique was adopted to prepare solid lipid nanoparticles in an aqueous system using all-trans retinoic acid (ATRA) as a model drug. Physicochemical proterties of SLN were investigated in detail. Drug release from two sorts of ATRA-SLN was investigated using a dialysis bag method. Compared with ATRA solution, the in vivo pharmacokinetics of two sorts of ATRA-SLN after intravenous injection to rats were studied.</p><p><b>RESULTS</b>Solid lipid nanoparticles loaded with all-trans retinoic acid was readily and quickly prepared by ultrasonic technique. The morphological investigation by Transmission Electron Microscopy (TEM) showed that the particles had round and uniform shapes. The mean diameters of them were (158 +/- 9) nm and (89 +/- 11) nm separately. The SLN dispersion was stable at 4 degrees C for more than one year. Drug loading was 3.3%, drug entrapment efficiency was more than 95%, the in vitro release was well in accordance with Weibull distribution. Compared with ATRA control solution, SLN could stay in the blood circulation for a longer time after intravenous injection.</p><p><b>CONCLUSION</b>The ultrasonic technique was appropriate for the preparation of solid lipid nanoparticles.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Taille de particule
/
Polysorbates
/
Trétinoïne
/
Science des ultrasons
/
Vecteurs de médicaments
/
Pharmacocinétique
/
Chimie
/
Systèmes de délivrance de médicaments
/
Rat Wistar
/
Poloxamère
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2005
Type:
Article
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