Effect of crocin on intracellular calcium concentration in cultured bovine aortic smooth muscle cells / 药学学报
Acta Pharmaceutica Sinica
; (12): 778-781, 2004.
Article
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| WPRIM
| ID: wpr-241400
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>AIM</b>To study the effect of crocin on intracellular calcium concentration ([Ca2+]i) in cultured bovine aortic smooth muscle cells (BASMCs).</p><p><b>METHODS</b>Cells were loaded with fluorescence probe Fluo-3/AM and [Ca2+]i was measured by laser scanning confocal microscope (LSCM).</p><p><b>RESULTS</b>In the presence or absence of extracellular Ca2+, crocin (1 x 10(-8), 1 x 10(-7), 1 x 10(-6) mol x L(-1)) concentration-dependently inhibited the [Ca2+]i elevation induced by 1 x 10(-2) mol x L(-1) H2O2 (for the former, the inhibition rates were 34.1%, 57.1% and 74.3%, while for the latter were 26.2%, 32.1%, 50.0%). In the absence of extracellular Ca2+, crocin (1 x 10(-8), 1 x 10(-7), 1 x 10(-6) mol x L(-1)) could inhibit the [Ca2+]i elevation induced by 70 mmol x L(-1) CHCl3, the inhibition rates were 27.8%, 27.8% and 50.0% respectively.</p><p><b>CONCLUSION</b>Crocin could inhibit the extracellular Ca2+ influx and release of intracellular Ca2+ stores in endoplasmic reticulum.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Aorte thoracique
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Pharmacologie
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Plantes médicinales
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Caroténoïdes
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Cellules cultivées
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Chimie
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Chloroforme
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Calcium
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Biologie cellulaire
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Myocytes du muscle lisse
Limites du sujet:
Animals
langue:
En
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2004
Type:
Article