The invasion ability and expressions of matrix metalloproteinase-13 and calcium-binding protein S100A4 are inhibited by hyperthermia in human Tca8113 cells / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 655-659, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-241879
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of hyperthermia on anti-invasion of Tca8113 and the expression change of matrix metalloproteinase-13 (MMP-13) and calcium-binding protein S100A4 (S100A4).</p><p><b>METHODS</b>Tca8113 cell pools were incubated at 43 degrees C for 0, 40, 80, 120 min, respectively, and at 37, 41, 43, 45 degrees C respectively for 80 min. The effect of high temperatures on the invasion ability of Tca8113 was measured in vitro. The slides of cells were made and incubated at 43 degrees C for 0, 40, 80, 120 min, respectively. Immunocytochemical method was employed for detecting the expression change of MMP-13 and S100A4 protein. Tca8113 cells were incubated at 43 degrees C for 0, 40, 80, 120 min respectively and at 37, 41, 43, 45 degrees C respectively for 80 min. Western blot method was conducted for detecting the expressionchange of MMP-13 and S100A4 protein.</p><p><b>RESULTS</b>As incubating time at higher temperature lasted, the proportion of the cells with invasion ability decreased. Except groups of 40 min and 80 min at 43 degrees C and 41, 43 degrees C for 80 min, the rest groups show significant statistic differences (P < 0.05). The expression intensity of MMP-13 and S100A4 proteins in Tca8113 cells would decrease as incubating time at higher temperature lasted. The content of MMP-13 and S100A4 proteins would decrease as incubating time at higher temperature lasted or incubating temperature increased. Except the groups of 40, 80 min at 43 degrees C and 41, 43 degrees C for 80 min, statistic differences were identified (P < 0.05).</p><p><b>CONCLUSION</b>The invasion of Tca8113 could be inhibited by hyperthermia. The mechanism of this effect may be due to protein expression inhibition of MMP-13 and S100A4.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines de liaison au calcium
/
Protéines S100
/
Lignée cellulaire tumorale
/
Matrix Metalloproteinase 13
/
Hyperthermie provoquée
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
West China Journal of Stomatology
Année:
2011
Type:
Article
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