Gene detection of severe acute respiratory syndrome-related coronavirus / 中华病理学杂志
Chinese Journal of Pathology
; (12): 212-214, 2003.
Article
de Zh
| WPRIM
| ID: wpr-242198
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a newly real-time RT-polymerase chain reaction assay for severe acute respiratory syndrome (SARS) related coronavirus in human whole blood.</p><p><b>METHODS</b>A pair of primers and a probe (molecular beacon) had been designed that were specific for the recognition of a highly conservative region between 15 301 and 15 480 of the SARS-related coronavirus polymerase gene sequences obtained from GenBank (G130027616).</p><p><b>RESULTS</b>In the real-time RT-PCR assay, the extent of SARS related coronavirus amplification was measured in terms of the increase in fluorescence during the amplification process. The 145 bp fragment of PCR product was further confirmed by conventional PCR assay and proved by DNA sequencing to be identical to the target sequence to which the probe was hybridized.</p><p><b>CONCLUSION</b>This assay has a broad application for clinical diagnosis and surveillance investigation.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Données de séquences moléculaires
/
Séquence nucléotidique
/
RT-PCR
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Syndrome respiratoire aigu sévère
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Virus du SRAS
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Diagnostic
/
Génétique
Type d'étude:
Diagnostic_studies
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Pathology
Année:
2003
Type:
Article