An XcmⅠ-generated T vector and its applications in synthetic biology / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 956-965, 2016.
Article
Dans Chinois
| WPRIM
| ID: wpr-242284
ABSTRACT
For more economical and efficient DNA clonging, pFL-XS-T, a Biobrick-T vector was constructed based on pMD18-T vector, carrying clonging regions of XbaⅠ-XcmⅠ-XcmⅠ-SpeⅠ. The results revealed that PCR products could be conveniently inserted into pFL-XS-T vevtor digested by XcmⅠby means of TA cloning. The positive frequency of recombination can meet the experimental requirements and all the plasmids obtained meet Biobrick standard. Moreover, the pFL-XS-T is compatible with other Biobrick parts, and serves as a vector for functional DNA fragments screening.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Plasmides
/
ADN
/
Réaction de polymérisation en chaîne
/
Clonage moléculaire
/
Biologie synthétique
/
Vecteurs génétiques
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2016
Type:
Article
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