Construction and fermentation control of reductive TCA pathway for malic acid production in Saccharomyces cerevisiae / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1484-1493, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-242463
ABSTRACT
Malic acid is widely used in food, and chemical industries. Through overexpressing pyruvate carboxylase and malate dehydrogenase in pdc1-deficient Saccharomyces cerevisiae, malic acid was successfully produced through the reductive TCA pathway. No malic acid was detected in wild type Saccharomyces cerevisiae, however, 45 mmol/L malic acid was produced in engineered strain, and the concentration of byproduct ethanol also reduced by 18%. The production of malic acid enhanced 6% by increasing the concentration of Ca2+. In addition, the final concentration reached 52.5 mmol/L malic acid by addition of biotin. The increasing is almost 16% higher than that of the original strain.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Oxydoréduction
/
Pyruvate carboxylase
/
Saccharomyces cerevisiae
/
Microbiologie industrielle
/
Transduction du signal
/
Cycle citrique
/
Voies et réseaux métaboliques
/
Fermentation
/
Génie métabolique
/
Génétique
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2013
Type:
Article
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