Heterozygous genotypes and molecular characteristics of Organophosphorus resistance associated esterase B2 genes of Culex pipiens complex / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 390-394, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-242644
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the heterozygous genotype and molecular characteristics of Organophosphorus resistance associated with heterozygous Estbeta2 of esterase B2 gene from natural population of Culex pipiens complex.</p><p><b>METHODS</b>Genomic DNA was extracted from natural populations of Culex pipiens complex in Hangzhou. The PCR-restriction fragment length polymorphism (PCR-RFLP) assay was applied to type the resistance associated esterase gene. Estbeta2 of esterase B2 gene was identified by PCR-RFLP, and the genotyping for heterozygous Estbeta2 was carried out after restriction enzyme digesting by Bfm I endonuclease.</p><p><b>RESULTS</b>The DNA was isolated from 207 Culex pipiens respectively, while 156 PCR samples showed positive and the positive rate was 75.36% (156/207). The PCR-RFLP assay of esterase B2 gene revealed that the Estbeta2 was accounted about 28.20% (44/156) in 156 positive samples. There were two genotypes identified, namely homozygous Estbeta2 (90.90%, 30/33) and heterozygous Estbeta2 (9%, 3/33), heterozygous Estbeta2 was in existence of a hybrid form as which combined with Estbeta2 and a subtype (Estbeta2/Estbeta2(1)).</p><p><b>CONCLUSION</b>Heterozygous Estbeta2 of Organophosphorus resistance associated with esterase genotype was determined in natural population of Culex pipiens, and a genotyping method was established.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Composés organiques du phosphore
/
Pharmacologie
/
Phénotype
/
Résistance aux insecticides
/
Serine endopeptidases
/
Gènes d'insecte
/
Culex
/
Génétique
/
Génotype
/
Hétérozygote
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Preventive Medicine
Année:
2009
Type:
Article
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