Using the stable HSPA1A promoter-driven luciferase reporter HepG2 cells to assess the overall toxicity of coke oven emissions / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 883-887, 2012.
Article
de Zh
| WPRIM
| ID: wpr-242744
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Using the stable HSPA1A (HSP70-1) promoter-driven luciferase reporter HepG2 cells (HepG2/HSPA1A cells) to assess the overall toxicity of coke oven emissions.</p><p><b>METHODS</b>The stable HepG2/HSPA1A cells were treated with different concentrations of coke oven emissions (COEs) collected from the top, side, and bottom of a coke oven battery for 24 h. After the treatments, luciferase activity, cell viability, malondialdehyde (MDA) concentration, Olive tail moment, and micronuclei frequency were determined, respectively.</p><p><b>RESULTS</b>The bottom COEs induced significant increases (P < 0.01) in relative luciferase activity up to 1.4 times the control level at 0.15 µg/L. The low dose of side COEs (0.02 µg/L) led to a significant increase (P < 0.01) in relative luciferase activity that progressively increased to 2.1 times the control level at 65.4 µg/L. The top COEs produced a strong dose-dependent induction of relative luciferase activity up to over 5 times the control level at the highest concentration tested (202 µg/L). In HepG2/HSPA1A cells treated with the bottom COEs, relative luciferase activity was positively correlated with MDA concentration (r = 0.404, P < 0.05). For the three COEs samples, positive correlations were observed between relative luciferase activity and Olive tail moment and micronuclei frequency.</p><p><b>CONCLUSION</b>The relative luciferase activity in HepG2/HSPA1A cells can sensitively reflect the overall toxicity of COEs. The stable HepG2/HSPA1A cells can be used for rapid screening of the overall toxicity of complex air pollutants in the workplace.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Exposition professionnelle
/
Régions promotrices (génétique)
/
Coke
/
Gènes rapporteurs
/
Tests de toxicité
/
Protéines du choc thermique HSP70
/
Micronoyaux à chromosomes défectueux
/
Toxicité
/
Cellules HepG2
/
Génétique
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Année:
2012
Type:
Article