Saturated fatty acid induces insulin resistance partially through nucleotide-binding oligomerization domain 1 signaling pathway in adipocytes / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 211-217, 2013.
Article
Dans Anglais
| WPRIM
| ID: wpr-243188
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the potential role of nucleotide-binding oligomerization domain 1 (NOD1), a component of the innate immune system, in mediating lipid-induced insulin resistance in adipocytes.</p><p><b>METHODS</b>Adipocytes from Toll-like receptor 4 deficiency mice were used for stimulation experiments. The effect of oleate/palmitate mixture on nuclear factor-κB (NF-κB) activation was analyzed by reporter plasmid assay. The release of proinflammatory chemokine/cytokines production was determined by using real-time PCR. Insulin-stimulated glucose uptake was measured by 2-deoxy-D-[3H] glucose uptake assay. Chemokine/cytokine expression and glucose uptake in adipocytes transfected with small interfering RNA (siRNA) targeting NOD1 upon fatty acids treatment were analyzed.</p><p><b>RESULTS</b>Oleate/palmitate mixture activated the NF-κB pathway and induced interleukin-6, tumor necrosis factor-α, and monocyte chemoattractant protein-1 mRNA expressions in adipocytes from mice deficient in Toll-like receptor 4, and these effects were blocked by siRNA targeting NOD1. Furthermore, saturated fatty acids decreased the ability of insulin-stimulated glucose uptake. Importantly, siRNA targeting NOD1 partially reversed saturated fatty acid-induced suppression of insulin-induced glucose uptake.</p><p><b>CONCLUSION</b>NOD1 might play an important role in saturated fatty acid-induced insulin resistance in adipocytes, suggesting a mechanism by which reduced NOD1 activity confers beneficial effects on insulin action.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Physiologie
/
Insulinorésistance
/
Transduction du signal
/
Facteur de transcription NF-kappa B
/
Adipocytes
/
Récepteur de type Toll-4
/
Protéine adaptatrice de signalisation NOD1
/
Acides gras
/
Métabolisme
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Chinese Medical Sciences Journal
Année:
2013
Type:
Article
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