Generation of transgene-free induced pluripotent stem cells with non-viral methods / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 50-54, 2013.
Article
Dans Anglais
| WPRIM
| ID: wpr-243219
ABSTRACT
Induced pluripotent stem (iPS) cells were originally generated from mouse fibroblasts by enforced expression of Yamanaka factors (Oct3/4, Sox2, Klf4, and c-Myc). The technique was quickly reproduced with human fibroblasts or mesenchymal stem cells. Although having been showed therapeutic potential in animal models of sickle cell anemia and Parkinson's disease, iPS cells generated by viral methods do not suit all the clinical applications. Various non-viral methods have appeared in recent years for application of iPS cells in cell transplantation therapy. These methods mainly include DNA vector-based approaches, transfection of mRNA, and transduction of reprogramming proteins. This review summarized these non-viral methods and compare the advantages, disadvantages, efficiency, and safety of these methods.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Transduction génétique
/
Transfection
/
Transgènes
/
Reprogrammation cellulaire
/
Cellules souches pluripotentes induites
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Chinese Medical Sciences Journal
Année:
2013
Type:
Article
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