Construction of spider silk functional platform via intein trans-splicing / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1704-1714, 2016.
Article
Dans Chinois
| WPRIM
| ID: wpr-243687
ABSTRACT
To provide technical support for spider silk functional modification, we developed a simple and efficient functional platform via intein trans-splicing. Small ubiquitin-related modifier protein (SUMO) was fused to the recombinant spider silk protein (W2CT) by peptide bond via S0 split intein Ssp DnaB trans-splicing, resulting in a protein SUMOW2CT. However, incorporation of exogenous protein led to mechanical property defect and lower fiber yield, and also slowed down the fiber assembly velocity but no obvious differences in supercontraction and chemical resistance when compared with fibers from W2CT (W). SUMO protease digestion showed positive results on the fibers, indicating that the SUMO protein kept its native conformation and bioactive. Above all, this work provides a technical support for spider silk high simply and efficient functionalized modification.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Araignées
/
Protéines recombinantes
/
Chimie
/
Épissage des protéines
/
Épissage en trans
/
Petites protéines modificatrices apparentées à l'ubiquitine
/
Soie
/
Intéines
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2016
Type:
Article
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