Expression of CD66c (CEACM6) in adult acute leukemia and its significance / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 370-373, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-243945
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of CD66c (CEACM6) in adult acute leukemia and its significance.</p><p><b>METHODS</b>Acute leukemia cell lines HL-60, K562, LCL721.221 and Jurkat were cultured in vitro. RT-PCR and multi-parameter flow cytometry were applied to analysis of CD66c mRNA and protein expression respectively in the cell lines and patient' s bone marrow leukemic cells. Cytogenetic analysis for 199 bone marrow samples from leukemia patients and Minimal Residual Disease (MRD) detection for 25 CD66c positive B lineage ALL were performed.</p><p><b>RESULTS</b>(1) CD66c expression both on cell surface and in plasma were negative in all the cell lines. (2) Four of 127 AML (3.15%) (mainly of M2 and M4), and 28 of 79 ALL (35.44%) (all of B linage ALL) were CD66c positive the subtypes of the ALL being common B-ALL (20/54) and pre B-ALL (8/11) including 8 Ph + B-linage ALL. (3) Six-month relapse rate was significantly different between the MRD positive and negative patients. (4) CD66c mRNA was strongly expressed in B-linage ALL. For the cell lines, only the HL60 cells weakly expressed CD66c mRNA.</p><p><b>CONCLUSION</b>CD66c expression could be a useful bio-marker for the MRD analysis in ALL, and is closely associated with its transcription level.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
ARN messager
/
Leucémie aigüe myéloïde
/
Antigène carcinoembryonnaire
/
Antigènes CD
/
Molécules d'adhérence cellulaire
/
Maladie résiduelle
/
Cellules HL-60
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Cellules K562
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Leucémie-lymphome lymphoblastique à précurseurs B et T
/
Protéines liées au GPI
Limites du sujet:
Adolescent
/
Adulte
/
Adulte très âgé
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2006
Type:
Article
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