Nifedipine attenuates vascular inflammation via inhibin NF-κB activity / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 1025-1030, 2010.
Article
de Zh
| WPRIM
| ID: wpr-244074
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects and related mechanism of nifedipine on vascular inflammation induced by cuff placement.</p><p><b>METHODS</b>Adult male C57BL/6J mice (10 to 12 weeks of age) were assigned to control (no cuff placement without nifedipine), cuff placement (cuff placement without nifedipine) and treatment (cuff placement with nifedipine 1 or 5 mg×kg(-1)×d(-1)) groups. Activity of NF-κB in injured artery was measured 5 days after operation. MCP-1 expression and nuclear translocation of NF-κB were examined in injured artery 7 days after operation.</p><p><b>RESULTS</b>DNA-binding activity of NF-κB was significantly increased in the injured artery 5 days after cuff placement which could be downregulated by nifedipine 5 mg×kg(-1)×d(-1). MCP-1 mRNA expression in the injured arteries was increased 7 days after cuff placement and which could be significantly attenuated by nifedipine 5 mg×kg(-1)×d(-1). Cuff placement decreased the cytoplasmic level of p50, IκBα, IκBβ, and increased the nuclear level of p50. Nifedipine 5 mg×kg(-1)×d(-1) significantly attenuated these changes.</p><p><b>CONCLUSION</b>Our results suggest that high dose nifedipine could suppresses expression of MCP-1 induced by injured arteries via the inhibin NF-κB DNA binding activity, thereby attenuating vascular inflammation.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Maladies vasculaires
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Vaisseaux sanguins
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Nifédipine
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Facteur de transcription NF-kappa B
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Chimiokine CCL2
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Inflammation
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Métabolisme
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Souris de lignée C57BL
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Cardiology
Année:
2010
Type:
Article