Effect of ischemia preconditioning on pro- and anti- angiogenic molecule expression and functional arteries formation / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 819-822, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-244138
ABSTRACT
<p><b>OBJECTIVE</b>to observe the effect of ischemia preconditioning (IPC) on the expression of pro-angiogenic VEGF, PDGF and anti-angiogenic ADAMTS-1, and arteriogenesis.</p><p><b>METHODS</b>rat heart IPC model was made by 4 circles of occluding the LAD for 6 min followed by 6 min of reperfusion. The expression of VEGF, PDGF-B and ADAMTS-1 in the ischemic area was examined with immunohistochemistry at 6, 12 and 24 h after IPC. IPC plus myocardial infarction model was induced by LAD ligation 24 h after IPC, 14 days later, the anti-SM-α-actin antibody was used to detect the mature neovascularization in the border of the infracted area.</p><p><b>RESULTS</b>VEGF, PDGF-B and ADAMTS-1 were significantly upregulated in the ischemic area in IPC group compared with the control group (P < 0.05). Density of mature arteries was also significantly increased in IPC plus MI group than that in MI group (P < 0.05).</p><p><b>CONCLUSION</b>IPC promoted the formation of mature new arteries which may be modulated by upregulating VEGF, PDGF-B, and ADAMTS-1 expressions.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Artères
/
Régulation positive
/
Rat Sprague-Dawley
/
Néovascularisation physiologique
/
Préconditionnement ischémique
/
Protéines proto-oncogènes c-sis
/
Facteur de croissance endothéliale vasculaire de type A
/
Protéines ADAM
/
Protéine ADAMTS1
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Cardiology
Année:
2010
Type:
Article
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