Analysis of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid and its clinical significance / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVES</b>To study the essence of N-terminal amino acid sequence of 12 000-protein in gingival crevicular fluid (GCF).</p><p><b>METHODS</b>GCF samples from patients with RPP and AP were collected. 12 000-protein was separated by SDS-PAGE and transformed to PVDF by electronic transformation. The aim band was cut to be analyzed in 491 Protein Sequencer.</p><p><b>RESULTS</b>The first ten of N-terminal amino acid sequence of 12 000-protein in GCF was Met, Leu, Thr, Glu, Leu, Glu, Lys, Ala, Leu, Asn. Through checking up in MS-Edman, the sequence was similar to "Ca binding protein, MRP8" which is the light subunit of Calprotectin.</p><p><b>CONCLUSIONS</b>Calprotectin is a major protein in granulocytes and monocytes, and is related to many inflammatory diseases, maybe served as a effective marker for evaluating the inflammation of periodontium.</p>