Expression of rhBMP-7 gene in transduced bone marrow derived stromal cells / 中国医学科学杂志(英文版)

ABSTRACT

<p><b>OBJECTIVE</b>To explore the possibility of expression of exogenous gene in transduced bone marrow derived stromal cells (BMSCs).</p><p><b>METHODS</b>The marker gene, pbLacZ, was transferred into cultured BMSCs and the expression of transduced gene by X-gal staining was examined. Then plasmid pcDNA3-rhBMP7 was delivered to cultured BMSCs. Through immunohistochemical staining and RT-PCR assay, the expression of rhBMP7 gene was detected.</p><p><b>RESULTS</b>The exogenous gene could be expressed efficiently in transduced BMSCs.</p><p><b>CONCLUSION</b>The present study provided a theoretical basis to gene therapy on the problems of bone and cartilage tissue.</p>