Effect of beclin1 on vincristine-induced dopaminergic neurons injury in zebrafish / 药学学报
Acta Pharmaceutica Sinica
;
(12): 843-848, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-245005
ABSTRACT
To investigate vincristine-induced dopaminergic neurons toxicity and mechanism, and explore the molecular target to reduce the toxicity, zebrafish was chosen as a model animal, based on RT-PCR, Western blotting, whole mount in situ immunofluorescence and other technical means. The results showed that the transcription levels of tyrosine hydroxylase gene and dopamine transporter protein gene were inhibited. Furthermore, the number of dopaminergic neurons was decreased by vincristine. Autophagy was suppressed and beclin1 gene expression was inhibited in a dose-dependent manner by vincristine in larval zebrafish. Up-regulated beclin1 partly reduced vincristine-induced neurotoxicity, and down-regulated beclin1 increased toxicity. Beclin1 plays an important role in vincristine-induced dopaminergic neurons toxicity.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Autophagie
/
Tyrosine 3-monooxygenase
/
Vincristine
/
Danio zébré
/
Régulation négative
/
Régulation de l'expression des gènes
/
Protéines de poisson-zèbre
/
Relation dose-effet des médicaments
/
Protéines régulatrices de l'apoptose
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2014
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS