Dystrophin detection by immunofluorescent technique for diagnosing muscular dystrophy / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 239-242, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-245324
ABSTRACT
<p><b>OBJECTIVE</b>To establish a specific technique for diagnosing and classifying Duchenne muscular dystrophy (DMD), Becker muscular dystrophy (BMD), facioscapulohumeral muscular dystrophy (FSHD) and neurologic dystrophy.</p><p><b>METHODS</b>Forty-seven cases were detected by immunofluorescence technique for analyzing dystrophin located in skeletal muscle cell membrane with the use of mouse monoclonal antibodies, goat and rabbit polyclonal antibodies.</p><p><b>RESULTS</b>The normal individuals showed ringed positive staining stripe around muscle fibers. Negative result of staining was seen in 16 DMD patients. Eleven BMD patients had discontinuous or a patchy positive staining pattern, and all of 10 FSHD and 10 neurological amyotrophic patients showed positive dystrophin staining.</p><p><b>CONCLUSION</b>Detecting dystrophin in the skeletal muscle cell membrane of muscular patients is an efficient technique for diagnosing and classifying various types of muscular dystrophy.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Chimie
/
Dystrophine
/
Technique d'immunofluorescence
/
Muscles squelettiques
/
Myopathie de Duchenne
/
Dystrophie musculaire facio-scapulo-humérale
/
Diagnostic
/
Diagnostic différentiel
/
Métabolisme
Type d'étude:
Etude diagnostique
Limites du sujet:
Adolescent
/
Adulte
/
Enfant
/
Enfant d'âge préscolaire
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Medical Genetics
Année:
2002
Type:
Article
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