Role of silencing PRL-3 expression by miRNA interference in the growth of gastric cancer / 中华外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the role of silencing PRL-3 expression by miRNA interference in gastric cancer growth.</p><p><b>METHODS</b>RNA interference mediated by recombinant lentivirus expressing artificial PRL-3 miRNA was employed to knockdown PRL-3 expression in human SGC7901 gastric cancer cells. MTT assay and tumor implantation experiment were conducted to determine the role of PRL-3 in the proliferation of SGC7901 cells and the tumor growth.</p><p><b>RESULTS</b>Transfection of recombinant lentivirus expressing artificial PRL-3 miRNA significantly suppressed the proliferation of SGC7901 cells in vitro. The implanted tumor size of the PRL-3 transfection group was (1.92 +/- 0.18) cm3, significantly smaller than those in control groups [(4.74 +/- 0.39) cm3] (P < 0.05).</p><p><b>CONCLUSIONS</b>Silencing of PRL-3 significantly suppressed the proliferation of SGC7901 cells and tumor growth in vivo. PRL-3 could be a potential therapeutic target in gastric cancer.</p>