A new molecular method to authenticate radix trichosanthis as well as its adulterants and substitutes / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore a new molecular method to authenticate Radix Trichosanthis.</p><p><b>METHOD</b>Three 20 mer primers based on the ITS sequence was designed. The PCR reaction system was optimized and applied to nineteen different sources of Radix Trichosanthis and nine adulterants and substitutes.</p><p><b>RESULT</b>Polymorphic map of Radix Trichosanthis and its adulterants was obtained from primer TKS1-64. 560 bp and 960 bp bands were authentic markers for Radix Trichosanthis.</p><p><b>CONCLUSION</b>Primer TKS1-64F possesses the advantages of good stability and reproducibility. This new method is named as anchored primer amplification polymorphism DNA(APAPD). It was a potential method to used in molecular identification of other meteria medica.</p>