Synthesis, identification of artificial antigen of catalpol and preliminary study of immunogenicity / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1287-1290, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-246109
ABSTRACT
The method of monoclonal antibody-based immunoassay has a great importance in the study of quality control of traditional Chinese medicine (TCM) and detection of trace components in vivo animals. Synthesis of small molecule artificial antigen is the prerequisite for the establishment of this method. In present study, catalpol-BSA was synthesized by sodium periodate oxidation method. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry ( MALDI-TOF-MS) and molecular exclusion chromatography showed that catalpol was successfully conjugated with BSA. The mice could specifically produce anti-catalpol antibodies with titer up to 18000. The artificial antigen of catalpol was successfully synthesized.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Sérumalbumine bovine
/
Dosage immunologique
/
Chimie
/
Allergie et immunologie
/
Glucosides d'iridoïdes
/
Médecine traditionnelle chinoise
/
Souris de lignée BALB C
/
Anticorps
/
Antigènes
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2015
Type:
Article
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