Characterization of hepatitis B virus antigen expression in vitro and in vivo transduced by different transfer plasmids carrying HBV infectious genome / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 96-98, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-246170
ABSTRACT
<p><b>OBJECTIVE</b>To character HBV antigen expression in vitro and in vivo transduced by different transgenic plasmids carrying infectious genome of hepatitis B virus (HBV).</p><p><b>METHODS</b>We constructed four different lentiviral transfer plasmids (carrying 1.3 full-length genome of HBV, by replacing the EGFP express box in pCS-CG plasmid with HBV genome and with different structural element, named as pCS-HBV1.3 (pCS-HBV1.3 X, pCS-HBV1.3 P, pCS-HBV1.3 N and pCS-HBV1.3 K). We detected the expression of HBsAg and HBeAg by ELISA in different time after transfected Huh 7 cells or hydrodynamic injection into C57 BL/6 mice with transfer plasmids pCS-HBV, respectively.</p><p><b>RESULTS</b>We detected significant expression of HBsAg over 5 days after transfected Huh 7 cells (in vitro) or hydrodynamic injection into C57 BL/6 mice (in vivo) with transfer plasmids pCS-HBV1.3 X, pCS-HBV1.3 P and pCS-HBV1.3 K. The expression level and dynamics of HBsAg and HBeAg in the sera of mice is consistent with that of in the supernatant of Huh-7 cell. Furthermore, the expression of HBV antigens were modulated by the direction and position of HBV insert, also by some lentiviral vector cis-elements (cPPT and RRE).</p><p><b>CONCLUSION</b>The optimal lentiviral transfer plasmids (pCS-HBV1.3 X, pCS-HBV1.3 P and pCS-HBV1.3 K) could be further used for establishment and application of HBV transgenic cell or animal model.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Plasmides
/
Transduction génétique
/
Génome viral
/
Lentivirus
/
Modèles animaux de maladie humaine
/
Génétique
/
Antigènes de l'hépatite virale B
/
Méthodes
/
Souris de lignée C57BL
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2012
Type:
Article
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