Construction of recombinant plasmid expressing S1 gene of new type of reovirus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 361-363, 2011.
Article
de Zh
| WPRIM
| ID: wpr-246239
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct the recombinant plasmid containing S1 gene of new type of reovirus, and to study the expression of protein sigma1 in Vero cells.</p><p><b>METHODS</b>The recombinant plasmid, named pC-S, was constructed by cloning S1 gene into vector pCAGGS/MCS. Then Vero cells were transfected with pC-S and collected at 24, 48, 72 h post transfection followed by SDS-PAGE and Western-Blot assay.</p><p><b>RESULTS</b>Results both SDS-PAGE and Western-Blot assay indicated that sigma1 protein could be expressed well and the highest expression level was 72 h post transfection.</p><p><b>CONCLUSIONS</b>Sigma1 protein could be expressed well in Vero cells by transfected with recombinant plasmid containing S1 gene, and could give some implications for subsequent research on virus-host interactions.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Plasmides
/
Reoviridae
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Protéines virales
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Cellules Vero
/
Protéines recombinantes
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Expression des gènes
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Chlorocebus aethiops
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Génétique
/
Métabolisme
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Experimental and Clinical Virology
Année:
2011
Type:
Article