Study on the counting of Streptococcus mutans, Streptococcus sanguis, Haemophilus actinomycetemcomitans by methyl thiazolyl tetrazolium colorimetric method / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 306-310, 2010.
Article
de Zh
| WPRIM
| ID: wpr-246597
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of methyl thiazolyl tetrazolium (MTT) colorimetric method and the applied condition for the normal bacteria in the mouth, as Streptococcus mutans (S. mutans), Streptococcus sanguis (S. sanguis), Haemophilus actinomycetemcomitans (H. actinomycetemcomitans).</p><p><b>METHODS</b>Colony forming units (CFU) which was the standard antitheses was used to count bacteria. This study would gain some parameters by changing wavelength, reactive time, dosage and so on. MTT colorimetric method was applied in the counting of S. mutans, S. sanguis and H. actinomycetemcomitans.</p><p><b>RESULTS</b>When counting S. mutans, the best wavelength was 510 nm, the best range was 1.5 x 10(5) - 1.0 x 10(7) CFU x mL(-1). When counting S. sanguis, the best wavelength was 545 nm, the best range was 1.5 x 10(5) - 2.0 x 10(7) CFU x mL(-1). When counting H. actinomycetemcomitans, the best wavelength was 557 nm, the best range was 1.0 x 10(6) - 5.0 x 10(7) CFU x mL(-1). MTT colorimetric method can be used for different aged S. mutans, S. sanguis and H. actinomycetemcomitans.</p><p><b>CONCLUSION</b>Oral bacteria could be counted by MTT colorimetric method, which is fast and convenient.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Salive
/
Streptococcus mutans
/
Streptococcus sanguis
/
Bactéries
/
Aggregatibacter actinomycetemcomitans
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
West China Journal of Stomatology
Année:
2010
Type:
Article