Expression of Osterix mRNA and protein levels in cultured human periodontal ligament cells after mechanical stimulation / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 214-228, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-246619
ABSTRACT
<p><b>OBJECTIVE</b>To examine the expression of Osterix (Osx) mRNA and protein after application of mechanical force on human periodontal ligament cells (HPDLCs), and to investigate the role of Osx in orthodontic alveolar bone remodeling.</p><p><b>METHODS</b>HPDLCs were isolated and cultured in vitro with explant method. Approximately 2.5 x 10(5) cells were seeded onto six-well cell culture plates and then were exposed to centrifugal force for 1, 2, 4, 6, 8 or 12 h at 631 r x min(-1). The expression of Osx mRNA and protein was measured by reverse transcription-polymerase chain raction (RT-PCR) and Western blot respectively. Immunofluorescence assay was used to detect the expression and subcellular At the initial time point, Osx mRNA had a weak exlocalization of Osx protein by green fluorescence.</p><p><b>RESULTS</b>pression and protein was not detected. Under the mechanical stimulation, both mRNA and protein levels of Osx were upregulated in a time-dependent manner. Furthermore, Osx protein was translocated gradually from the cytosol into the cell nuclei.</p><p><b>CONCLUSION</b>The expression and activation of Osx were enhanced by mechanical stress in HPDLCs, which indicates that Osx may play an important role in HPDLCs osteogenic differentiation and periodontal tissue remodeling induced by mechanical stress.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéogenèse
/
Desmodonte
/
Contrainte mécanique
/
ARN messager
/
Différenciation cellulaire
/
Lignée cellulaire
/
Remodelage osseux
/
Techniques de culture cellulaire
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
West China Journal of Stomatology
Année:
2010
Type:
Article
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